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發(fā)布時間：2018/7/27
點擊次數(shù)：514
BioChain是高質量加工生物樣品產品和分析服務的先進者，用于推進轉化醫(yī)學，伴隨診斷和臨床產品研發(fā)。BioChain的產品可用于DNA和RNA測序，PCR和RT-PCR，基因表達分析，DNA和RNA純化，蛋白質提取和純化以及蛋白質表達分析。BioChain的下一代測序一站式服務，從臨床樣品采集，樣品制備，測序到生物信息學分析，將*改變行業(yè)。
除了我們*的生物標本和樣品制備業(yè)務，BioChain正在進入臨床應用領域，為癌癥和生殖健康提供臨床基因組學診斷產品和臨床試驗服務。BioChain致力于通過持續(xù)流程改進計劃以競爭力的價格實現(xiàn)高質量的產品和服務。BioChain建立了全面的質量保證計劃，以滿足客戶的滿意度，并為我們的內部生產設施保持ISO 9001：2015和ISO 13485：2003認證。
cfPure® Cell-Free DNA Extraction Kit cfure無細胞DNA提取試劑盒是一種基于磁性珠的DNA提取試劑盒，旨在從人血漿和血清中分離循環(huán)無細胞DNA(Cfdna)。該工具包有一個快速，簡單，和自動 省略友好協(xié)議，允許用戶快速提取高質量的cfdna，準備用于下游應用，包括實時PCR、數(shù)字PCR和下一代測序。 該試劑盒也是一個強有力的工具液體活檢研究，如早期發(fā)現(xiàn)腫瘤，無創(chuàng)產前檢查等。
Biochain K5011625MA
概觀
BioChain的cfPure™無細胞DNA純化試劑盒專為快速有效分離無循環(huán)細胞DNA（cfDNA）而設計。該試劑盒專門設計用于5mL或更大體積的樣品。
描述
BioChain的cfPure®無細胞DNA提取試劑盒專為快速有效純化無循環(huán)細胞DNA（cfDNA）而設計。該系統(tǒng)利用二氧化硅涂層的順磁顆粒來純化無細胞DNA，從小于1 mL到大于10 mL的血清或血漿。開發(fā)緩沖液以確保有效回收100bp-500bp的DNA片段，以大限度地回收無細胞DNA?；厥盏腄NA適用于廣泛的下游應用，例如亞硫酸氫鹽測序，NGS和qPCR。
從血漿，血清和尿液中提取高質量的無細胞DNA，用于新一代測序（NGS），亞硫酸氫鹽測序，PCR，qPCR，ddPCR和其他要求苛刻的應用
•用途廣泛： 從單管或24至96孔板中提取<1mL至> 10 mL血清或血漿
•自動化友好： 在KingFisher™和液體處理儀器上，提取大約需要50分鐘
•時間效率： 手動提取協(xié)議只需不到75分鐘
•成本效益： 從1 mL血漿中每次提取的競爭價格<$ 3（250 mL試劑盒的定價）
•洗脫體積： 范圍為15μL至50μL
cfPure®協(xié)議快速簡便，允許用戶在一小時或更短的時間內處理多個樣品，使其成為生物標記物篩選的理想選擇。BioChain開發(fā)了自己的二氧化硅涂層磁珠技術，旨在在分離過程中有效回收低分子量無細胞DNA。除了易于操作和易于自動化之外，如果您正在凈化少量等離子體，您可以使用更少的磁珠溶液 - 節(jié)省您的資金。成功分析cfDNA的關鍵是確保有效純化較小的DNA片段（100-500 bp）。如圖1所示，cfPure®無細胞DNA提取試劑盒可有效回收這些重要的小片段。
Cfpure旨在成為可擴展的套件（圖2）。這意味著您可以僅使用cfDNA提取所需的珠子和試劑的數(shù)量。這也意味著每個試劑盒的提取次數(shù)取決于您希望從中純化DNA的樣品量。例如，如果您正在使用1mL樣品，我們的100mL試劑盒（目錄號K5011610）可處理多達100個樣品，我們的250 mL試劑盒（目錄號K5011610）可處理250個樣品。如果您正在使用10mL樣品，我們的100mL試劑盒（目錄號K5011610）可處理多達10個樣品，我們的250 mL無細胞DNA提取試劑盒（目錄號K5011625）可處理25個樣品。
與用于分離cfDNA的其他技術相比，磁珠提供許多益處。珠子比玻璃纖維過濾器更有效地結合DNA，從而產生更高和更一致的產量。另外，由于不使用過濾器和真空歧管，因此在提取過程中不存在細胞顆粒堵塞這些物品的風險。這種堵塞問題特別涉及富含蛋白質的大體積樣品，例如cfDNA研究中常用的血漿。
cfPure®fCRDNA提取試劑盒提取的cfDNA非常適用于NGS等下游應用。在第三方研究中，使用BioChain的cfPure®（B），競爭對手T（T）和競爭對手Q（Q，基于柱）的cfDNA提取試劑盒從4個供體的10ml人血漿中提取cfDNA。然后為每個樣品構建文庫，并進行全基因組測序。從數(shù)據(jù)分析來看，三種提取方法并未顯示出顯著的性能差異：插入大?。▓D3），定位率，全基因組覆蓋率，替代率和GC偏差（圖4）。
我們優(yōu)化的基于磁珠的協(xié)議使用戶能夠擴展協(xié)議，適用于100μl至10 ml的任何樣品體積，具有高度可重復的結果和有效的cfDNA回收率（表1和2）。
cfPure®CellFree DNA提取試劑盒適用于KingFisher™（圖5）和液體處理儀器。使用KingFisher™Flex和BioChain的cfPure®無細胞DNA提取試劑盒可在50分鐘內提取多達96個1 ml樣品，或在1小時內提取24個5 ml樣品。
biochain 2018年部分產品列表：
K5011610 | cfPure® Cell Free DNA Extraction Kit (100 ml) | 1 Kit (100 ml) | biochain |
K5011625 | cfPure® Cell Free DNA Extraction Kit (250 ml) | 1 Kit (250 ml) | biochain |
K5011625MA | cfPure® MAX Cell-Free DNA Extraction Kit | 1 Kit (250 ml) | biochain |
Z1510001 | SeqPure PCR Purification Kit (5 ml) | 5 ml | biochain |
Z1510002 | SeqPure PCR Purification Kit (50 ml) | 50 ml | biochain |
Z1510003 | SeqPure PCR Purification Kit (500 ml) | 500 ml | biochain |
L5011001 | PureSil-Silica Magnetic Beads | 1 L | biochain |
L5011010 | PureSil-Silica Magnetic Beads | 10 mL | biochain |
Z1322006 | AnaPrep Bacterial DNA Extraction Kit (48) For extracting genomic DNA from bacteria | 1 kit (48 extractions) | biochain |
Z1322002 | AnaPrep Blood DNA Extraction Kit 1200 (48) For extracting genomic DNA from mammalian whole blood, peripheral mononuclear cell, or buffy coat. Sample volume range: up to 1 ml | 1 kit (48 extractions) | biochain |
Z1322001 | AnaPrep Blood DNA Extraction Kit 200 (48) For extracting genomic DNA from mammalian whole blood, peripheral mononuclear cell, or buffy coat. Sample volume range: up to 400 µl | 1 kit (48 extractions) | biochain |
Z1322005 | AnaPrep Cultured Cell DNA Extraction Kit (48) For extracting genomic DNA from up to 5×105 cultured cells | 1 kit (48 extractions) | biochain |
Z1322009 | AnaPrep FFPE DNA Extraction Kit (48) For extracting genomic DNA from formalin-fixed paraffin-embedded tissue (FFPE) samples | 1 kit (48 extractions) | biochain |
Z1322010 | AnaPrep Forensic DNA Extraction Kit (48) For extracting genomic DNA from a wide range of forensic and human identity samples | 1 kit (48 extractions) | biochain |
Z1322007 | AnaPrep HPV DNA Extraction Kit for swab samples (48) For extracting HPV DNA from swab samples | 1 kit (48 extractions) | biochain |
Z1322008 | AnaPrep TB DNA Extraction Kit (48) For extracting Mycobacterium tuberculosis DNA from sputum | 1 kit (48 extractions) | biochain |
Z1322004 | AnaPrep Tissue DNA Extraction Kit (48) For extracting genomic DNA from a variety of animal tissues, swab, and blood stains | 1 kit (48 extractions) | biochain |
Z1322015 | AnaPrep Total RNA Extraction Kit | 1 kit | biochain |
Z1322017 | AnaPrep Total RNA Extraction Kit with DNase-Treatment | 1 kit | biochain |
Z1322003 | AnaPrep Viral Nucleic Acid Extraction Kit (48) For extracting viral nucleic acids from plasma | 1 kit (48 extractions) | biochain |
Z1322011 | AnaPrep Viral/Pathogen Nucleic Acids Extraction Kit A | 1 kit | biochain |
Z1322012 | AnaPrep Viral/Pathogen Nucleic Acids Extraction Kit B | 1 kit | biochain |
T2235090-SB | CancerSeq™ Paraffin Tissue Tumor Block: Colon | 1 Block | biochain |
T2235152-SB | CancerSeq™ Paraffin Tissue Tumor Block: Lung | 1 Block | biochain |
T2235086-ST | CancerSeq™ Paraffin Tissue Tumor Slides: Breast | 5 Slides | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235086-SB | CancerSeq™ Paraffin Tissue Tumor Block: Breast | 1 Block | biochain |
T2235218A-SB | CancerSeq™ Paraffin Tissue Tumor Block: Skin Melanoma | 1 Block | biochain |
T2235090-ST | CancerSeq™ Paraffin Tissue Tumor Slides: Colon | 5 Slides | biochain |
T2235152-ST | CancerSeq™ Paraffin Tissue Tumor Slides: Lung | 5 Slides | biochain |
T2235218A-ST | CancerSeq™ Paraffin Tissue Tumor Slides: Skin Melanoma | 5 Slides | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
Z1510001 | SeqPure PCR Purification Kit (5 ml) | 5 ml | biochain |
Z1510002 | SeqPure PCR Purification Kit (50 ml) | 50 ml | biochain |
Z1510003 | SeqPure PCR Purification Kit (500 ml) | 500 ml | biochain |
L1271010 | T4 RNA Ligase 1 | 1000 Units | biochain |
L1271050 | T4 RNA Ligase 1 | 5000 Units | biochain |
L1271500 | T4 RNA Ligase 1 | 50000 Units | biochain |
L1270010 | T4 RNA Ligase 2, truncated | 1000 Units | biochain |
L1270500 | T4 RNA Ligase 2, truncated | 50000 Units | biochain |
KS072012-I | Aligner 1-12 | 12 | biochain |
KS072012-II | Aligner 13-24 | 1 Kit | biochain |
KS072012-III | Aligner 25-36 | 1 Kit | biochain |
KS072012-IV | Aligner 37-48 | 1 Kit | biochain |
KS071012-12 | Gel Breaker Tubes | 12 | biochain |
KS071012-11 | Gel Cutter | 12 | biochain |
KS071012-13 | Gel Filter | 12 | biochain |
KS073012-I | RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 1-12 | 1 Kit | biochain |
KS073012-II | RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 13-24 | 1 Kit | biochain |
KS073012-III | RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 25-36 | 1 Kit | biochain |
KS073012-IV | RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 37-48 | 1 Kit | biochain |
KS073012 | RapidSeq High Yield Directional mRNA Sample Prep Kit – Without Aligner | 1 Kit | biochain |
KS073012-T | RapidSeq High Yield Directional mRNA Sample Prep Kit, Trial version enough for 2 rxn. Customer select aligners | 1 Kit | biochain |
KS074012-I | RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 1-12 | 1 Kit | biochain |
KS074012-II | RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 13-24 | 1 Kit | biochain |
KS074012-III | RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 25-36 | 1 Kit | biochain |
KS074012-IV | RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 37-48 | 1 Kit | biochain |
KS074012 | RapidSeq High Yield Small RNA Sample Prep Kit – Without Aligners | 1 Kit | biochain |
KS074012-T | RapidSeq High Yield Small RNA Sample Prep Kit, Trial version enough for 2 rxn. Customer select aligners | 1 Kit | biochain |
K5017100 | Blood and Serum DNA Isolation Kit | 1 kit | biochain |
K5011610 | cfPure® Cell Free DNA Extraction Kit (100 ml) | 1 Kit (100 ml) | biochain |
K5011625 | cfPure® Cell Free DNA Extraction Kit (250 ml) | 1 Kit (250 ml) | biochain |
K5011625MA | cfPure® MAX Cell-Free DNA Extraction Kit | 1 Kit (250 ml) | biochain |
K5019100 | FFPE Tissue DNA Extraction Kit | 1 kit | biochain |
K5011350 | FFPE Tissue DNA Extraction Kit – Column | 1 kit | biochain |
K5011450 | FFPE Tissue DNA Extraction Kit — Magnetic Beads | 1 kit | biochain |
K5016005 | Genomic DNA Extraction Kit | 1 kit | biochain |
K5011050 | Saliva DNA Isolation Kit | 1 kit | biochain |
K5018100 | Serum DNA Isolation Kit | 1 kit | biochain |
K5011250 | Stool DNA Isolation Kit | 1 kit | biochain |
K5011150 | Urine DNA Isolation Kit | 1 kit | biochain |
Z5060009 | xMag-Streptavidin Microparticles | 1 ml | biochain |
T6234701-1 | FDA Standard Frozen Tissue Array – Human Adult Normal | 2 slides | biochain |
T6234701-2 | FDA Standard Frozen Tissue Array – Human Adult Normal | 4 slides | biochain |
T6334701-1 | FDA Standard Frozen Tissue Array – Mouse Normal | 2 slides | biochain |
T6334701-2 | FDA Standard Frozen Tissue Array – Mouse Normal | 4 slides | biochain |
T6434701-1 | FDA Standard Frozen Tissue Array – Rat Normal | 2 slides | biochain |
T6434701-2 | FDA Standard Frozen Tissue Array – Rat Normal | 4 slides | biochain |
T6235201 | Frozen Tissue Array – 24 Different Prostate Tumors and 4 Corresponding Normal Controls | 1 slide | biochain |
T6235086-1 | Frozen Tissue Array – 37 Different Breast Tumors and 3 Corresponding Normal Controls | 1 slide | biochain |
T6235086-5 | Frozen Tissue Array – 37 Different Breast Tumors and 3 Corresponding Normal Controls | 5 slides | biochain |
T6235090-1 | Frozen Tissue Array – 37 Different Colon Tumors and 3 Corresponding Normal Controls | 1 slide | biochain |
T6235090-5 | Frozen Tissue Array – 37 Different Colon Tumors and 3 Corresponding Normal Controls | 5 slides | biochain |
T6235142-1 | Frozen Tissue Array – 37 Different Kidney Tumors and 3 Corresponding Normal Controls | 1 slide | biochain |
T6235142-5 | Frozen Tissue Array – 37 Different Kidney Tumors and 3 Corresponding Normal Controls | 5 slides | biochain |
T6235183-1 | Frozen Tissue Array – 37 Different Ovarian Tumors and 3 Corresponding Normal Controls | 1 slide | biochain |
T6235183-5 | Frozen Tissue Array – 37 Different Ovarian Tumors and 3 Corresponding Normal Controls | 5 slides | biochain |
T6235152-1 | Frozen Tissue Array – 40 Different Lung Tumors and 5 Corresponding Normal Controls | 1 slide | biochain |
T6235152-5 | Frozen Tissue Array – 40 Different Lung Tumors and 5 Corresponding Normal Controls | 5 slides | biochain |
T6734700-G02-1 | Frozen Tissue Array – Dog Female Adult Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6734700-G02-5 | Frozen Tissue Array – Dog Female Adult Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6734700-G01-1 | Frozen Tissue Array – Dog Male Adult Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6734700-G01-5 | Frozen Tissue Array – Dog Male Adult Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6234700-1 | Frozen Tissue Array – Human Adult Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6234700-5 | Frozen Tissue Array – Human Adult Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6235700-1 | Frozen Tissue Array – Human Tumor and Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6235700-5 | Frozen Tissue Array – Human Tumor and Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6834700-G02-1 | Frozen Tissue Array – Rabbit Female Adult Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6834700-G02-5 | Frozen Tissue Array – Rabbit Female Adult Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6834700-G01-1 | Frozen Tissue Array – Rabbit Male Adult Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6834700-G01-5 | Frozen Tissue Array – Rabbit Male Adult Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6134035-1 | Multiple Species Frozen Tissue Array – Brain | 1 slide | biochain |
T6134035-5 | Multiple Species Frozen Tissue Array – Brain | 5 slides | biochain |
T6134149-1 | Multiple Species Frozen Tissue Array – Liver | 1 slide | biochain |
T6134149-5 | Multiple Species Frozen Tissue Array – Liver | 5 slides | biochain |
上海起發(fā)實驗試劑有限公司是實驗試劑一站式采購服務商
1：強大的進口輻射能力，血清、抗體、耗材、大部分限制進口品等。
2：產品種類齊全，經營超過700多個品牌，基本涵蓋所有生物實驗試劑耗材。
3：提供加急服務，貨品一般1-2周到貨。
4：富有競爭力的價格優(yōu)勢，絕大部分價格有優(yōu)勢。
5：多年積累良好的信譽，大部分客戶提供貨到付款服務?？蛻舭ㄇ迦A、北大、交大、復旦、中山等100多所高校，ROCHE，阿斯利康、國藥、fisher等藥企。
6：我們還是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech；sysy,TriLink BioTechnologies,Inc；worthington-biochem,zyagen等幾十家國外公司授權代理。
7：我們還是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等*批發(fā)，歡迎合作。
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